public object in the Global ASCX file
How long will an object in the Global ASCX file persist? For the life of the application?
Do you mean Global.asax? Web applications are stateless; the Global class gets re-instantiated on every request. You may store data in the Application object if you want it to exist for the life of the app, or in the Session object if you want it to exist for the duration of an individual user session.
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